Abstract: The effects of chemical and organic fertilizers on glandular trichome density of oriental tobacco lamina from different stalk positions were experimented.The results showed that the density of multicell-headed trichome with long stalk was affected greatly by fertilizer type,that of monocellheaded trichome with long stalk was affected to a certain extent,and that of trichome with short stalk was influenced a little.The trichome density in upper and middle leaves was influenced more greatly than that in lower leaves by fertilizer type.Organic fertilizer obviously increases the density of multicellheaded trichome with long stalk and total trichome density of upper leaves,and chemical fertilizer increases the density of multicell-headed trichomes with long stalk of middle leaves.