Abstract: A method based on ultrasonic extraction combined with fast high performance liquid chromatography for simultaneously determining phenol,cresol,and dihydroxybenzene in mainstream cigarette smoke (MS) was developed.The total particulate matter collected on Cambridge filter pad was extracted with 1% acetic acid under ultrasonic for 20 minutes.The extract was filtrated with 0.45 μm filter membrane,then separated and detected by HPLC with a fast separation column and a fluorescence detector.The volatile phenols in MS of 10 brands of domestic Virginia type and 10 brands of blended type cigarettes were determined with this method.The results indicated that:1) the recoveries of the method were from 97.9% to 100.3% with RSD of 1.92%-4.62% and the limit of detection of 5.74 to 14.70 ng/cig,the analyzing took 6 minutes;2) the contents of dihydroxybenzenes and phenol were much higher in MS of Virginia type cigarettes than in MS of blended type cigarettes,while the content of total cresols was slightly higher in MS of Virginia type cigarettes;and 3) in MS of same type cigarettes,the volatile phenols in order of content were p-dihydroxybenzene> o-dihydroxybenzen> phenol> m, o-cresol> p-cresol> m-dihydroxybenzene.The method is suitable for the fast analysis of volatile phenols in batches of MS samples.