钙信号在烟草普通花叶病高温隐症中的作用

Effects of Ca²⁺ in Symptom Masking of TMV Inoculated Tobacco Under High Temperature Stress

摘要: Ca²⁺和抑制剂处理结果表明,烟草接种TMV后经Ca²⁺处理再高温胁迫,能够明显提高烟草叶片的过氧化物酶(POD)和超氧化物歧化酶(SOD)活性,而经Ca²⁺特异性螯合剂EGTAO,O'-Bis(2-aminoethyl)ethyleneglycol-N,N,N',N'-tetraacetic acid和钙调素的特异性抑制剂氯丙嗪(CPZ)处理能够显著降低烟草叶片的POD和SOD活性。对各个处理的表现型观察发现,经Ca²⁺及EGTA和CPZ处理后的烟草与对照(清水)相比,病毒的潜育期不存在明显的差异,但经Ca²⁺处理的烟草发病程度比清水处理稍轻,EGTA和CPZ处理的烟草发病程度比对照重,说明Ca²⁺信号途径可能参与了高温胁迫下的症状隐蔽。

Abstract: The results of Ca²⁺ and inhibitor treatments showed that after TMV inoculating,the activities of POD and SOD in tobacco leaf increased obviously by Ca²⁺ treatment and followed by high temperature stress,while those activities decreased significantly by Ca²⁺ specific chelator EGTA and CaM specific inhibitor CPZ treatment.There was no significant difference in TMV latent period between different treatments(Ca²⁺,EGTA,CPZ,and check);however,mosaic symptom was slightly masked by Ca²⁺ treatment and aggravated by treatments by EGTA and CPZ;it indicated that Ca²⁺ signal route probably involved in symptom masking under high temperature stress.