Abstract: In order to increase the detectable number and test speed of organochlorine pesticide residue in tobacco, the standard method for determination of organochlorine pesticide residue in tobacco from ISO 4389:2000(GB/T 13596-2004) was improved. Soxhlet extraction and n-hexane (extracting solvent) were replaced by accelerated solvent extraction (ASE) and component solvent of w-hexane and acetone (1:1, v/v) f respectively, and extracting temperature was optimized. The residues of organochlorine pesticides in 65 domestic and imported tobacco samples were determined with the improved method and ISO 4389:2000 method, the results showed that:1) β -endosulfan was detected in 10 samples by the improved method, but not found by ISO 4389:2000; 2) the organochlorine pesticides detectable by both methods were almost the same except for β-endosulfan, quantitatively, their coefficients of variation were below the acceptable limits for trace pesticide determination of 20%; 3) no organochlorine pesticide was detectable in 17 samples t which accounted for 26.2% of total samples; 4) the detection rate of o, p' -DDT was the highest (up to 43. 1%), neither Dieldrin nor Endrin was detected in all samples;5) the numbers of detectable organochlorine pesticides were 47 and 43 in domestic and imported cigarette samples, respectively; organochlorine pesticides were detected in all of the imported samples, particularly α-endosulfan, β-endosulfan, and o, p'一DDT,while these pesti-cides were detectable only in 4 domestic samples; 6) the numbers of detectable pesticides in domestic leafsamples were slightly higher than that in imported samples, which happened mainly in a couple of domestic samples; 7) the residues of organochlorine pesticides in all samples were lower than CORESTA recommendedlimits. The improved method could replace ISO 4389:2000 in fast detection of the residues of organochlorinepesticides in tobacco samples.