Abstract: The chlorine content in Hunan flue-cured tobacco and its effects on smoking quality were analyzed. The results showed that there was no significant difference between the chlorine contents in tobacco grown in different areas of Hunan, the chlorine content was low in general with only a few exceptions. Correlation analysis indicated that chlorine was significantly positively correlated to aftertaste and significantly negatively correlated to ash color. The smoking quality index in order of grey incidence degree with chlorine was irritation > offensive odor > ash color > combustibility > aftertaste > taste > impact > total score > aroma quality > flavor > aroma quantity > concentration. The effects of chlorine contents on smoking quality differed, lower chlorine content would reduce aroma, taste and deteriorate smoking quality. Proper chlorine supply once every two years in the areas where chlorine content in tobacco leaf was lower was recommended.