Abstract: By using distilled water as solvent and microwave and ultrasonic as assistant methods,the nicotine extracting technology was studied with orthogonal experiments.The results showed that:the factors in order of their effects on nicotine yield were power> extraction> time> ratio of liquid to solid,and the effects of power in the two assistant methods were extremely significant.The optimal extracting conditions were:1) microwave method:power 230W,extraction time 70 seconds and ratio of liquid to solid 40:1; 2) ultrasonic method:power 400w,extraction time 25 minutes and ratio of liquid to solid 80:1.The extracting yield by microwave method was higher than that by ultrasonic method,and it was 11 times higher than that by distilled water alone under the same time,temperature and ratio of liquid to solid conditions.