Abstract: Ten fatty acid flavors of C₅ to C₁₄ chain length were injected into cigarettes respectively,and their distribution and transfer behavior were analyzed after the cigarettes were stored at room temperature for 7 days by simultaneous distillation and extraction combined with GC and GC/MS.The results showed that:1) the retention ratios in cut tobacco,loss ratios,and immigration ratios to filter tip of the ten fatty acids ranged from 30.34% to 56.67%,42.34% to 68.15% and 0.17% to 1.50%,respectively;2) the transfer ratios to mainstream smoke and the interception ratios by filter tip of the ten fatty acids ranged from 0 to 17.26% and 2.85% to 13.87%,respectively;3) the transfer ratios to mainstream smoke of 8 homologous acids from valeric to tetradecanoic acids increased with the increase of molecular weight,however,their retention ratios in cut tobacco,loss ratios,immigration ratios to filter tip and interception ratios by filter tip changed irregularly;4) as for valeric acid and isovaleric acid,3-methylvaleric acid and hexanoic acid,the retention ratios of n-fatty acids in cut tobacco were higher than those of iso-fatty acids,while their loss ratios in cut tobacco,transfer ratios to mainstream smoke,immigration ratios to filter tip and interception ratios by filter tip were lower than those of iso-fatty acids.