Abstract: The inheritance of resistance to tobacco black shank was analyzed with an invitro anther cultured doubled haploid(DH) population derived from F₁ plants of the cross between burley tobacco varieties Burley37(P₁,resistant variety) and Burley67(P₂,susceptible variety).Black shank incidences were investigated in the P₁,P₂ and DH population inoculated with race 0 Phytophthora parasitica var.nicotianae in 2 consecutive years,and disease indexes were calculated.The resistance to black shank was analyzed with the major gene and polygene mixing inheritance model on the basis of plant's quantitative character,and other genetic parameters of the DH population were also estimated.The results showed that black shank incidence and disease index were controlled by 2 pairs of complementary major genes plus polygene(E-1-7) and 2 pairs of equally additive major genes plus polygene(E-1-3),respectively.Heritability values of major genes were 54.14% and 50.68%,and those of polygenes were 26.60% and 19.31%,the number of efficiency factor of polygenes was 0.61 and 1.31,respectively.Therefore,the resistance to black shank of burley tobacco was conditioned dominantly by 2 pairs of major genes and polygenes.The heritance of resistance to tobacco black shank in tobacco cultivars is relatively stable in breeding practice.However,environmental factors,including temperature and humidity,also have some influences on the occurrence and epidemic of tobacco black shank.