Abstract: The effects of several elicitors, including mannitol, cellulase, pectinase, salicylic acid, eggplant verticillium wilt and tomato fulvia fulva on tobacco (NC89) cell growth and synthesis of coenzyme Q₁₀ were compared. The results showed that proper concentration of mannitol was benefit to NC89 cell growth, but it would inhibit the intracellular synthesis of coenzyme Q₁₀;when the concentration of mannitol was 5.0 mg/L, cell's dry weight increased by 14.6%, while the production of coenzyme Q₁₀ only increased by 1.9%. Salicylic acid, pectinase and tomato fulvia fulva promoted the intracellular synthesis of coenzyme Q₁₀ though certain inhibition to tobacco cell growth. When the concentrations of salicylic acid and tomato fulvia fulva were 5.0 mg/L and 25 mL/L respectively, the content of intracellular coenzyme Q₁₀ increased by 25.8% and 66.1%, but the production of coenzyme Q₁₀ decreased by 7.0% and 1.9% respectively. The content and production of coenzyme Q₁₀ could be improved by 54.0% and 40.0% respectively with the concentration of pectinase at 3 U/L. Eggplant verticillium wilt elicitor and cellulase were beneficial to both cell growth and synthesis of intracellular coenzyme Q₁₀, when their concentrations were 25 mL/L and 2 U/L, the production of coenzyme Q₁₀ increased by 71.1% and 36.6%, respectively.