Abstract: Using flue-cured tobacco cv.K326 as material, the plant growth and leaf glandular trichomes development of flue-cured tobacco in the deficiency of nutrient elements during plant growing were studied.The results indicated that a deficiency of N, P, K, Mg not only negatively affected the growth of tobacco plant, but also had a significant effect on the growth and development of leaf glandular trichomes.N deficiency would decrease leaf area and stimulate the growth of various glandular trichomes, and the density of glandular trichome increased accordingly, while P, K, Mg deficiency would reduce the density of glandular trichome greatly besides leaf area reduction.Therefore, sufficient P, K, Mg nutrients application in the early growth stage of tobacco plant is particularly important for enhancing glandular trichome density and glandular secretion of tobacco leaves.