烟草法呢基焦磷酸合酶基因的克隆及序列分析

Molecular Cloning and Sequence Analysis of Farnesyl Pyrophosphate Synthase Gene in Tobacco

摘要: 采用逆转录-聚合酶链式反应(RT-PCR)方法,以烟草品种K326叶片总RNA为模板扩增出烟草法呢基焦磷酸合酶基因fps,克隆至载体PMD19-T中。序列分析表明,所克隆的cDNA序列全长702 bp,共编码234个氨基酸残基,其核酸序列与玉米、杜仲、拟南芥、青蒿、Nicotiana sanderae×Nicotiana langa-dorffii杂交种的法呢基焦磷酸合酶基因的核酸序列同源性分别为74%,76%,77%,80%和97%。

Abstract: Farnesyl pyrophosphate synthase gene,fps,was obtained via amplification by reverse transcription polymerase chain reaction(RT-PCR) with the total RNA of cv.K326 tobacco leaf as a template and cloned into vecter PMD19-T.Sequence analysis showed that the cloned cDNA was 702bp in length and encoded 234 amino acid residues.The homology between the sequence of its nucleic acid and that of the fps genes of Zea mays L.,Eucommia ulmoides Oliv.,Arabidopsis thaliana L.,Artemisia annua L.,Nicotiana sanderae×Nicotiana langadorffii was of 74%,76%,77%,80% and 97%,respectively.