高效液相色谱法测定卷烟纸中的6种有机酸根

Determination of Anions of Six Organic Acids in Cigarette Paper by HPLC

摘要: 建立了一种以0.005 mol/L硫酸为萃取剂、高效液相色谱法同时测定卷烟纸中柠檬酸根、酒石酸根、苹果酸根、乳酸根、琥珀酸根及富马酸根的方法,并采用该方法测定了18种卷烟纸样品。结果表明:①这6种有机酸根的平均回收率为91.64%~105.09%,相对标准偏差 ≤ 2.65%;②18种卷烟纸样品中柠檬酸根、酒石酸根、苹果酸根、乳酸根、琥珀酸根和富马酸根含量范围分别为6.03~24.45,0~8.13,0~6.91,0~0.98,0~0.31和0~0.1 mg/g。

Abstract: A method for simultaneously determining the citric,tartaric,malic,lactic,succinic,and fumaric anions in cigarette paper was developed by extraction with 0.005 mol/L sulfuric acid solution and separation by HPLC.18 cigarette paper samples were tested with the developed method,the results showed that:1) the average recoveries of the six organic acidic anions ranged from 91.64% to 105.09%,with RSDs of ≤ 2.65%;2) the contents of citric,tartaric,malic,lactic,succinic,and fumaric anions in samples ranged from 6.03 to 24.45,0 to 8.13,0 to 6.91,0 to 0.98,0 to 0.31,and 0 to 0.1 mg/g,respectively.