Abstract: In order to investigate their content difference in flue-cured tobacco of different cultivars,crop years and growing areas and the relationship between these elements,the contents of Mn,Fe,Cu,Zn,Ca and Mg in tobacco leaves of C₃F from different growing areas produced in 2004,2005,2007 and 2008 were determined and their correlations were analyzed.The results showed that:1) The CV of Cu and Mn,Fe and Zn,Mg and Ca were 60%,40% to 50%,30%,respectively,Ca had the least CV and Cu the largest.The average CV of the 6 elements was the highest for cv.K326 lines(48.86%) and lowest for cv.CuiBi lines(24.82%).2) The contents of the 6 elements in leaves were obviously different between different crop years and dependent on their cultivar and growing area as well.The elements' contents in flue-cured tobacco were obviously related to growing areas.The Fe,Ca and Mg contents in samples from Huanghuai tobacco growing areas were higher,while samples from Southeast China tobacco growing areas featured lower Fe,Ca,Mg contents and higher Zn content.The Mn and Cu contents in samples from upper-and middle reaches of the Yangtze River and Southwest China tobacco growing areas were higher.3) The contents of Ca and Mg in leaf extremely significantly positively correlated to Fe content,while extremely significantly negatively correlated to Mn and Zn contents.