Abstract: In order to find out the effects of climatic factors on leaf trichome exudates,a field experiment on flue-cured tobacco cv.Yun 85 was conducted at Longgang of Kaiyang County,Weining County and Tianzhu County in Guizhou Province.Ripe leaves harvested from lower,middle and upper stalk positions were fluecured,and the major surface substances were extracted with methylene chloride and analyzed.The results showed that the contents of major components in trichome exudate differed significantly between leaves grown under different climatic conditions.The contents of major trichome exudate in leaves from Tianzhu were considerably higher than those from Weining and Longgang;and the difference between Tianzhu and Longgang leaves reached an extremely significantly.The contents of different trichome exudates varied greatly among leaves grown under different climatic conditions,β-cembratriene-diol had the biggest variation,its average variation coefficient reached 114.28%;while retinol had the least average variation coefficient of 40.66%.For different soils,the climate affected the contents of trichome exudates in different ways,it indicated that there existed certain interactions between factors of climate and soil.