烤烟八氢番茄红素合成酶基因的克隆与序列分析

Cloning and Sequence Analysis of T-psy1 Gene from Flue-cured Tobacco

摘要: 利用同源性克隆方法,从烤烟栽培品种K326(Nicotiana tobacum K326)中克隆获得烤烟八氢番茄红素合成酶基因的全长cDNA序列(命名为T-psy1),GenBank中的登录号为HM345582,该基因全长为1521bp,编码的蛋白含441个氨基酸,蛋白登录号为ADK25054。Blast搜索结果及进化分析结果表明,该基因序列与观赏烟草、番茄和辣椒的八氢番茄红素合成酶基因同源性分别为95%,91%,86%。其编码蛋白与观赏烟草、番茄、牛奶子的PSY基因编码蛋白一致率分别为97%,90%和74%。

Abstract: Adopting a homology cloning method,the full length cDNA sequence of tobacco phytoene synthase gene(named T-psy1) was cloned from flue-cured tobacco cv.K326,the T-psy1 gene,GenBank accession number HM345582,included 1521bp,with coding protein containing 441 amino acids,protein accession number ADK25054.The results of BLAST search and phylogenetic analysis showed that T-psy1 presented high genetic homologies with the phytoene synthase of ornamental tobacco,tomato and pepper,which were 95%,91% and 86%,respectively;and the coding protein consistences of T-psy1 gene with phytoene synthase gene of ornamental tobacco,tomato and Elaeagnus umbellate were 97%,90% and 74%,respectively.