Abstract: A gas chromatography-mass spectrometry(GC/MS) method under selected ion monitoring(SIM) mode was developed for the simultaneous determination of 1,2-propylene glycol,glycerol and triethylene glycol in smokeless tobacco products(STPs) by using 1,4-butanediol as an internal standard.Thirty STP samples were parallel tested by GC/MS/SIM and GC/FID,a CORESTA recommended method,and the results were compared.The results showed that:1) The recoveries of 1,2-propylene glycol,glycerol and triethylene glycol ranged from 89.3% to 100.3% with the limits of detection of 1.25 to 2.75 μg/g and RSDs of 1.16% to 4.37%.2) The contents of 1,2-propylene glycol,glycerol,and triethylene glycol in the samples ranged from 0.011 to 40.817,0.024 to 39.044,0.012 to 0.099 mg/g,respectively.3)1,2-propylene glycol in 13 samples and glycerol in 14 samples were detected,and their contents in 9 of the samples were accurately determinable,however,triethylene glycol was undetectable in all samples by GC/FID.4)1,2-propylene glycol in 29 samples,glycerol in 28 samples and triethylene glycol in 21 samples were detected,their contents in 16,25 and 11 of the samples could be accurately determined by GC/MS/SIM method,respectively.5) The Pearson correlation coefficients between the contents of 1,2-propylene glycol in 13 samples and glycerol in 9 samples determined by GC/FID and GC/MS/SIM were over 0.99 probable value less than significant level of 0.05 and paired test t value over significant level of 0.05.It was concluded that the target analytes in the samples determined by GC/MS/SIM agreed with those by GC/FID,however,GC/MS/SIM method was more sensitive and accurate.GC/MS/SIM method is more suitable for the qualitative and quantitative determination of 1,2-propylene glycol,glycerol,and triethylene glycol in smokeless tobacco products.