Abstract: The influences of amount of digesting agents,including CuSO₄,K₂SO₄,and concentrated H₂SO₄,on the results in determining total nitrogen content in tobacco and tobacco product were investigated.The total nitrogen content in the samples was determined by an automatic steam distillation and titration device(automatic Kjeldahl nitrogen analyzer).The results showed that the average recoveries of nitrogen ranged from 97.90% to 103.85% with the coefficient of variation below 2% when the sample was digested by 0.4 g CuSO₄,6.0 g K₂SO₄,and 12 mL concentrated H₂SO₄.There is no significant difference between the test results of the mentioned method and YC/T161-2002 standard method.The proposed method is suitable to the rapid analysis of total nitrogen in tobacco and tobacco products.