Abstract: A method was presented for the analysis of dithiocarbamate pesticide residues in tobacco,in which tobacco was put into a closed-conical flask,the residues of dithiocarbamate pesticides in tobacco were extracted and hydrolyzed to CS₂ under ultrasonic conditions,then CS₂ trapped in a layer of iso-octane overlaying the reaction mixture was taken for gas chromatography-mass spectroscopy analysis.The recoveries ranged from 90.4% to 106.0% with the relative standard deviations(RSD) of 3.2% to 9.5% and the limit of quantification(LOQ) of 0.017 mg/kg.This method can be used for the fast analysis of the dithiocarbamate pesticide residues in tobacco.