Abstract: In order to develop a new natural cigarette flavor,Gracilaria lemaneiformis was extracted with supercritical CO₂.The extract was analyzed by GC/MS and applied in cigarette as a flavor.The results showed that:1) the optimum extraction conditions were:extraction pressure 15 MPa,extraction temperature 45℃;2)40 components in the extract were identified,which were mainly linoleic acid,(Z)-9-octadecenoic acid,palmitic acid,cinnamaldehyde,stearic acid,glyceryl linoleate;3) the offensive odor and irritation of cigarette smoke was obviously lowered,the sweetness of cigarette smoke was increased and the taste of cigarette was improved by the extract.