Abstract: In order to optimize the supercritical CO₂ extraction conditions for Hongda tobacco essence,the extraction yield was investigated by L₉(3⁴) orthogonal experiment,and the volatile components in the obtained extracts were analyzed by GC/MS,and the essence was applied into cigarette as a flavor.The results of the experiments indicated that the optimal extraction pressure,temperature,CO₂ flow rate and time were 30 MPa,45℃,25 kg/h and 2.0 hours,respectively.Thirty-six components were identified,and the main volatile components were:12isopropyl-1,5,9-trimethyl-4,8,13-cyclotetradecatriene-1,3-diol(21.28%),nicotine(15.88%),octadecyne(6.70%),2,5-epoxydihydroretroionone(4.49%),ledol(3.59%),1-methyl-4-(2-methyloxiranyl)-7-oxabicyclo4.1.0heptane(2.42%),ascaridole(2.32%),benzenamine(2.20%),4H-cyclopentadefphenanthrene(2.00%).The sensory evaluation showed that the essence obviously enhanced tobacco's natural aroma,and imparted a unique stylish character to tobacco.The optimized parameters increase the yield of essence,and the extraction process is feasible and stable.