Abstract: In order to investigate the effects of extraction methods on the composition of volatile oil from Huaiju chrysanthemum flower,the flower was extracted by steam distillation and enzymatic extraction respectively.The obtained volatile oils were analyzed by GC/MS and applied into cigarette.The results showed that:1) 41 and 36 components were respectively identified in the two volatile oils, including 26 and 21 known compounds; 10 of them were in common,mainly p-cymene, α-pinene, β-phellandrene and farnesol; the product by steam distillation was an oil-like blue liquid with a yield of 0.27%; product by enzymatic extraction was an oil-like light yellow liquid with a higher yield of 0.99%.2) the panel test results showed that the volatile oil from Huaiju flower could modify tobacco flavor,improve the smoothness,mildness and after taste,and reduce irritation of cigarette smoke.