烟草木质素的Fe-CA仿酶降解
Biomimetic Degradation of Lignin in Tobacco by Fe-CA System
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摘要: 为降低烟草中木质素的含量,提高再造烟叶的质量,制备了Fe-CA仿酶,并通过正交试验优化了烟草(烟梗和碎烟叶片)处理工艺。结果表明:①最优处理条件为:反应温度45℃,处理时间40 min,仿酶用量40 mmol/kg,在此条件下处理的烟草中木质素含量由3.56%降至1.69%,去除率达52.5%;②通过扫描电子显微镜(SEM)观察,处理后的烟草表面变得粗糙不平,有许多孔洞和凹坑,还有纤维骨架露出,表明仿酶可以高效脱除木质素;③仿酶处理后的再造烟叶的香气、杂气和协调性得到了改善,木质杂气减少,刺激性降低,品质得到提升。Abstract: In order to reduce the lignin content in tobacco and improve the quality of reconstituted tobacco,Fe-CA biomimetic system was prepared and the treatment process of tobacco(stem and scrap) was optimized by orthogonal experiment.The results indicated that the optimal condition was:temperature 45℃,treatment time 40 minutes,and application amount of Fe-CA biomimetic system 40 mmol/kg.The lignin content in tobacco decreased from 3.56% to 1.69%,and the removal rate reached 52.5%.SEM observation indicated that,the surface of treated tobacco became rough and scattered with many pores and holes,some fiber framework appeared.It was suggested that Fe-CA biomimetic treatment could reduce lignin in tobacco efficiently.Moreover,the sensory evaluation showed that the Fe-CA biomimetic treatment improved the aroma,harmony and quality of reconstituted tobacco and reduced its offensive odor and irritation.
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