Abstract: Taking K326,a flue-cured tobacco cultivar widely planted in Qujing,as a sample,the relationship of single leaf weight,length,width,stem content,and equilibrium moisture content of tobacco leaf with altitude, longitude and latitude where the tobacco was planted was studied with correlation analysis,path analysis and stepwise regression analysis. The results indicated that leaf length significantly positively correlated with longitude and extremely significantly negatively correlated with latitude;the correlation of leaf width with longitude was significantly positive, while with altitude was extremely significantly negative; single leaf weight extremely significantly negatively correlated with latitude;stem content extremely significantly positively correlated with latitude;equilibrium moisture content extremely significantly negatively correlated with longitude. Of all the physical properties,leaf length was susceptible to altitude,latitude and longitude the most. Of all the geographic factors,the influence of latitude was higher than the other factors on physical properties. The geographic factors influenced physical properties of tobacco mainly by direct effects,and assisted by indirect effects. There existed weak interactions among altitude,latitude and longitude. The multiple stepwise regression equation of leaf length, single leaf weight versus altitude,latitude and longitude was of significant level and fitted pretty well.