烟梗废料固态发酵生产苏云金芽孢杆菌的适宜条件筛选

Screening of Solid State Fermentation Conditions of Waste Stem for Producing Bacillus Thuringiensis

摘要: 以烟草废料烟梗为培养基主要原料,采用正交和单因素试验方法,对苏云金芽孢杆菌HD-1菌株(Bacillus thuringiensis HD-1)的固态发酵培养基及培养条件进行了优化。结果表明,烟梗固态发酵培养基的最佳组合为:烟梗88.13%,酵母粉1.17%,蛋白胨1.17%,豆饼粉5.88%,玉米粉2.93%,KH₂PO₄ 0.18%,MgSO₄·7H₂O 0.24%,FeSO₄0.12%和MnSO₄ 0.18%;最佳培养条件为:接种量6%,初始含水率65%,初始pH7.5。在这种发酵条件下的产孢量可稳定在9.5×10₁₀ CFU/g。因此,利用烟梗废料固态发酵生产苏云金芽孢杆菌是可行的。

Abstract: Using waste tobacco stem as raw material, the medium compositions and conditions of solid state fermentation for Bacillus thuringiensis HD-1 growth were optimized by orthogonal design and single factor experiments. The results showed that the optimal medium composition was: tobacco stem 88.13%, dusty yeast 1.17%, peptone 1.17%, ground soybean cake 5.88%, corn flour 2.93%, KH₂PO₄ 0.18%, MgSO₄·7H₂O 0.24%, FeSO₄ 0.12% and MnSO₄ 0.18%. The best culture conditions were: inoculation concentration 6%, initial water content 65%, initial pH of the medium 7.5, under which sporulation stabilized at 9.5×10₁₀ CFU/g. It was feasible to produce Bacillus thuringiensis by solid state fermentation using waste tobacco stem as the main medium．