Abstract: The robust flavor type flue-cured tobacco leaf from Sangzhi in Zhangjiajie was sliced into three portions, including tip, middle portion, and base portion. Their routine chemical components, thermal reaction and volatile constituents were analyzed by TG-DTA and GC-MS. On the basis of routine chemical components and volatile constituents, cluster analysis for whole leaf and each leaf portion was carried out. The results showed that: 1) the three portions differed significantly in terms of the contents of routine chemical components, the relative contents of volatile constituents and thermo-gravimetric loss rate. The nicotine content in the tip was the highest, while the contents of reducing sugar and total sugar in the tip and middle portion and the contents of potassium, chloride and total nitrogen in the base portion were higher. The contents of solaventone and palmitic acid in the tip, the contents of benzyl alcohol, geranyl acetone and myristic acid in the middle portion, and the contents of neophytadiene, 3-furaldehyde, megastigmatrienone and methyl linolenate in the base portion were higher. The thermal weight loss rate was highest in the middle portion and less in the base portion of the leaf. 2) Different portions of the leaf could be clustered into different categories. Each portion of tobacco leaf could be integrated into different formulation modules, whereby to widen the range of tobacco to be used in cigarette production.