Abstract: In order to study the quality properties and regional specialities of flue-cured tobacco in Jiangxi Province, the main chemical properties of tobacco leaves from 16 counties in Jiangxi Province were tested, evaluated and cluster analyses conducted. The results showed that: 1) The samples in which the level of potassium, phosphorus, nicotine, potassium/chloride ratio, reducing sugar, or total sugar/reducing sugar ratio matched that required by quality tobacco, accounted for 100%, 89.03%, 87.10%, 90.32%, 67.10% and 61.29%, respectively, of the total samples; the potassium contents in all samples were more than 2%, and the nicotine contents were all lower than 3.5%; the chemistry was suitable and harmonious by and large. 2) The differences of contents of total nitrogen, nicotine, reducing sugar and potassium, and sugar/alkali ratio among leaves from different stalk positions were significant and followed the general rule of distribution. 3) The leaves from Anfu, Huichang, Yongfeng, Taihe, Le'an and Xiajiang counties could be clustered into one category, in which reducing sugar content, sugar/alkali ratio and total sugar/reducing sugar ratio were relatively higher, while nicotine content were lower; the leaves from Ganxian, Zixi and Xingguo counties could be clustered into the second category, in which the contents of total nitrogen and nicotine, and total sugar/reducing sugar ratio were relatively higher, while sugar/alkali ratio were lower; the leaves from Guangchang, Shicheng, Xinfeng, Lichuan, Ruijin, Ningdu and Yihuang counties could be clustered into the third category, in which reducing sugar content and total sugar/reducing sugar ratio were relatively lower, and the other chemical indicators were moderate. The main chemical properties of flue-cured tobacco in Jiangxi Province possessed obviously regional specialities.