Abstract: SO₃^2-and SCN-were introduced into a system to improve the growth density of nano Au on tobacco mosaic virus (TMV) template. The suspension solution of the obtained composite was added into the filter of Kentucky reference cigarette to investigate its influence on CO in cigarette smoke. Transmission electron microscope,UV-visible absorption spectrometer and thermogravimetric analyzer were utilized to characterize the composite. The results showed that:1) at the application rate of 0.7 mL per cigarette,the reduction rate of CO delivery and selective removal rate of CO in cigarette smoke were 27.43% and 16.33%,respectively;2) the size of nano Au could be validly controlled and loading of nano Au could be more efficiently,which explained why the catalytic oxidization of CO in cigarette smoke was promoted.