Abstract: To find out the relationships between the mechanical properties of tobacco leaf and its moisture content, tobacco samples with 5 different moisture contents were prepared in a climate chamber, and the mechanical properties of C3F leaves from 5 areas (Huili in Sichuan, Xuchang in Henan, Mudanjiang in Heilongjiang, Dali in Yunnan and Zimbabwe) and B2F, C3F and X2F leaves from Guangyuan in Sichuan were analyzed. The results showed that:1) With the increase of moisture content, the adhesion, shearing force and tensile force of all samples increased first, then decreased. 2) For the samples of C3F from the 5 areas, the moisture content under which the adhesion reached its maximal value was higher than that corresponding to maximum shearing force and tensile force. 3) For B2F, C3F and X2F leaves from Guangyuan, the moisture contents corresponding to maximum adhesion, shearing force and tensile force varied in dependence on leaf grades.