废弃烟梗发酵生产深红酵母的工艺条件优化

Optimization of Fermentation Conditions for Producing Rhodotorula rubra JLC with Waste Tobacco Stems

摘要: 以废弃烟梗水提取液(Tobacco stem extraction,TSE)为主要基质,采用Plackett-Burman设计法对影响发酵生产富含类胡萝卜素的深红酵母(Rhodotorula rubra)JLC生物量的11个主要因子进行了筛选。结果表明:蔗糖、NaH₂PO₄、KH₂PO₄和培养时间对酵母生物量有显著影响,在Plackett-Burman试验中,JLC最大生物量干重为20.63 g/L。

Abstract: Plackett-Burman design was used to screen the major factors affecting fermentation production of a carotenoids-rich Rhodotorula rubra JLC from waste stems. The results showed that among the 11 tested factors, cane sugar, NaH₂PO₄, KH₂PO₄ and culture time significantly affected yeast yield. In Plackett-Burman experiments, the dry weight of maximum biomass reached 20.63 g/L.