Abstract: In order to effectively control the weeds in tobacco fields,the kind and population of weeds in seed bank of weeds in 0-25 cm plow layer soil sampled from five main tobacco growing areas of Shandong in 2010-2012 were investigated with germination method. The results showed that the population of monocotyledonous weeds was much higher than that of dicotyledonous weeds in most samples. The density of seed bank differed between growing areas,the highest density of 32891 seeds/m² was found in the sample from Miaoshan in Laiwu,while the lowest density of 1719 seeds/m² was found in the sample from Jiayue in Zhucheng. The density also differed between plow layers,generally,the largest was in the plow layer of 0-15 cm. Among monocotyledonous weeds,the populations of Cyperaceae and Gramineae were larger.