全烟气暴露与烟气冷凝物染毒方式的卷烟烟气体外微核测试结果比较

Comparison of Measurements from in vitro Micronucleus Assays for Cells Exposed to Whole or Condensate of Mainstream Cigarette Smoke

摘要: 采用VITROCELL^®系统建立了基于全烟气暴露的体外微核测试方法,并比较了全烟气暴露与烟气冷凝物染毒方式下卷烟主流烟气的体外微核测试结果。结果表明:该方法可以进行卷烟烟气的遗传毒性测试;在相同烟气剂量下,采用全烟气暴露方式测试的卷烟烟气遗传毒性大于烟气冷凝物染毒方式的测试结果;与传统的烟气冷凝物染毒方式相比,基于全烟气暴露方式的体外微核测试结果能够较全面地反映主流烟气的遗传毒性。

Abstract: An in vitro micronucleus assay method for whole smoke exposure of mainstream cigarette smoke was established with VITROCELL^{�/sup> system, and the measurements from in vitro micronucleus assays for cells exposed to whole or condensate of mainstream cigarette smoke were compared. The results indicated that the established method could be used for the genotoxicity testing of cigarette smoke. At the same smoke dosage, the genotoxicity of cigarette smoke determined with whole smoke exposure was greater than that with smoke condensate exposure. Comparing with traditional smoke condensate exposure, the measurements from in vitro micronucleus assay for whole smoke exposure relatively completely reflect the genotoxicity of mainstream cigarette smoke.}