Abstract: To determine the composition characteristics of stalks of different tobaccos and provide a sound basis for intensive development and utilization of tobacco stalk, the routine chemical, mineral and amino acid portions in stalks of flue-cured, burley, sun-cured, oriental and Maryland tobacco growing in Hubei Province were analyzed and compared. The results showed that they differed obviously. The stalk of burley tobacco was richer in reduce sugar, total sugar and total nitrogen, but lower in C/N; the stalk of flue-cured tobacco was richer in Ca, K, Mg and amino acids; the stalk of sun-cured tobacco was richer in chlorine, cellulose and higher in C/N, but poorer in Ca and Zn; the stalk of oriental tobacco was richer in P, Zn and total nitrogen, but poorer in nicotine, chlorine, amino acids (except for NH₃); the stalk of Maryland tobacco was richer in organic carbon, but poorer in reduce sugar, total sugar, K, B, Cu, Zn and cellulose.