Abstract: Abstract:A model for whole smoke-induced lung injury in Kunming mice was developed by referring to the procedures and methods of experimental models for tobacco smoke-induced lung cancer in mice at home and abroad. Sixty mice were randomly divided into two groups:the control group and the exposed group. The mice of exposed group were exposed to whole smoke within a vessel at the conditions that 10 cigarettes were smoked for 2 hours by smoking machine each day at the frequency of 1 puff/minute and 6 days per week. After 4 months of exposure, the exposed mice were dissected for pathological, immunohistochemical and proteomics analysis. The results showed that:1) Comparing with the control group, the lungs of exposed mice possessed distinct inflammatory cell infiltration, cilia lodging and deficiency, goblet cell hyperplasia of different degrees, alveolar cavities of different sizes, some alveolar septal became thinner or broken, and evident presence of sediment. 2) The positive rates of PCNA and p53 protein expression in exposed mice were 80.0% and 66.7%, respectively, which were significantly higher than those in the control group. 3) The expression of some water-soluble proteins lowered evidently in the lungs of exposed mice, the content of some protein bands with the molecular weight of 18.6-23.6 kD reduced obviously.