Abstract: In order to investigate the variation of polyphenols in the production of paper-making process reconstituted tobacco,three polyphenols,including chlorogenic acid,scopoletin and rutin,were analyzed by high performance liquid chromatography.And the effects of extraction temperature,ethanol concentration and concentration temperature on polyphenol contents were studied.The results showed that:1) The contents of the three polyphenols in tobacco dust and scrap were higher than in tobacco stem,however,the percentage of scopoletin in polyphenols was higher in tobacco stem.2) The base-sheet of reconstituted tobacco contained small amount of polyphenols,while a larger proportion of the polyphenols was washed out by white water.The effective utilization rates of chlorogenic acid,scopoletin,and rutin were 70.8%,47.1%,and 72.4%,respectively.3) Within the range from 40 to 90℃,higher temperature was beneficial to the extraction of polyphenols.The extraction efficiency of the polyphenols was the highest when the concentration of ethanol in the extractant was 70%.With the rise of concentration temperature,the contents of the polyphenols in extract reduced gradually.This study provided a reference for the regulation and control of polyphenols in the production of paper-making process reconstituted tobacco.