Abstract: The Myrrh essential oil was prepared with solvent-free microwave extraction method.Its physical properties were measured,the main functional groups were identified and the major components in the essential oil were analyzed by GC/MS.The application of the essential oil in tobacco flavoring was evaluated.The results showed that:1) The optimal extracting conditions were:grinding to 40 mesh,soaking for 3 hours,microwave power 800 W and extracting time 21 minutes,under which the yield of the extract was 3.95%.2) Sixty-two compounds were identified in the essential oil,including isolindestrene,curzerene,lindestrene,α-cedrene and β-elemene,etc.3) Myrrh essential oil could modify and improve cigarette smoke,reduce offensive odor effectively,enhance smoking taste and aftertaste,and reduce irritation.