Abstract: A method combining accelerated solvent extraction(ASE) with liquid chromatography-tandem mass spectrometry(LC-MS/MS) was developed for simultaneously determining 92 pesticide residues in tobacco,which integrated the extraction and purification of tobacco samples into one step by putting adsorbents at the bottom of extraction cell.The extract was analyzed by LC-MS/MS under multi-reaction monitoring(MRM) mode.The results showed that:1) The optimized extraction conditions were:acetonitrile as extraction solvent,4 g florisil and 40 mg graphitized carbon black(GCB) as adsorbents,extraction temperature 120℃,extraction pressure 10 MPa,static extraction time 6 minutes,flush volume 50% of extraction cell volume,static circulation twice.2) All matrix-matched calibration curves displayed good linearity(r²>0.99).The recoveries of standard addition at three levels of 0.1,0.5,and 1 mg/kg ranged from 70% to 124% with the relative standard deviations(RSDs) no more than 20%.The developed method is accurate,precise,and suitable for the determination of 92 pesticide residues in tobacco.