Abstract: To qualitatively and quantitatively analyze the oral irritation caused by cigarette smoke,a set of buccal cavity simulator was designed.By taking eight carbonyl compounds(formaldehyde,acetaldehyde,acetone,acrolein,propionaldehyde,crotonaldehyde,2-butanone and butyraldehyde) in cigarette smoke as research objects,the correlation of the eight carbonyl compounds in cigarette smoke with the irritation to tongue and palate in the simulated buccal cavity was analyzed through regulating the moisture content in cut tobacco via changing the equilibrious condition of cigarette,and coupling with cigarette's type.The results showed that:1) The distribution of the eight carbonyl compounds in cigarette smoke on the tongue was comparable to that on the palate in the simulated buccal cavity.2) The irritation to buccal cavity increased along with the increase of the carbonyl compounds in cigarette smoke as the moisture content in cut tobacco decreased.3) The simple correlation analysis results indicated that the detected levels of acetaldehyde,acetone,propionaldehyde,crotonaldehyde,2-butanone and butyraldehyde significantly correlated to oral irritation under standard conditions with the correlation coefficients higher than 0.81.