Abstract: To screen the genetic resources of flue-cured tobacco with high polyphenol content and investigate the differences of polyphenol content in tobacco of the same genotype under different ecological conditions,the polyphenol contents in flue-cured tobacco of 54 genotypes from Zhucheng in Shandong and Xichang in Sichuan were determined.The results showed that,both genotype and growing area ecological condition affected the polyphenol content in flue-cured tobacco greatly.The maximum absolute polyphenol content were 2 to 3 times as the minimum content in different genotypes,and the maximum relative chlorogenic acid and rutin contents were higher by 20 percentage points than that of the minimum.The varieties with the total polyphenol content higher than the average value in both areas included NC89,Jingyehuang,Zhongyan 102,Qinyan 95,Zhongyan 98,Yunyan 87,Zhongyan 14,Shanyu 2,Gexin 3,which could be used as the parent in high polyphenol content variety breeding.For the same genotype,the absolute contents of chlorogenic acid,rutin and total polyphenol in leaves from Sichuan were 1.5 to 2.0 times of those from Shandong;while the absolute scopoletin content in leaves from Shandong were nearly 2 times of those from Sichuan.Comparing with the leaves from Shandong,the polyphenol content in leaves from Sichuan featured higher absolute contents of chlorogenic acid and rutin and lower absolute content of scopoletin.