Abstract: To accurately evaluate the comprehensive trait of new flue-cured tobacco varieties(lines),the data of 12 trait indexes(including natural leaf number,yield,percentage of high grade leaf,grade index,nicotine content,reducing sugar content,potassium content,bacterial wilt disease index,climate spot disease index, appearance quality score,stem content and total score of sensory evaluation) obtained in 2012 Guizhou regional test were analyzed with fuzzy comprehensive evaluation method.GGE biplot analysis was used to analyze comprehensive evaluation results,conduct varieties(lines) evaluation and testing points evaluation,and analyze the adaptable areas of each variety(line).The results showed that Guiyan 2,2010A4 and MT-2 obtained better and stabler comprehensive evaluation value;YQ-1 obtained better comprehensive evaluation value in District 1(Longgang and Weining),as did Guiyan 2 in District 2(Zunyi,Guiding and Kaili) and 6517 in District 3(Yinjiang).According to evaluation of testing points,those in Guiding and Zunyi were good in representativeness and discrimination;those in Yinjiang,Weining and Longgang were good in discrimination, but not as good in representativeness;those in Kaili were good in representativeness,but not as good in discrimanation.