乳杆菌发酵烟梗制备生物塑料前体物L-乳酸的研究

Preparation of L-lactic Acid, A Precursor of Biological Plastic, by Fermentation of Waste Tobacco Stems with Lactobacillus

摘要: 为资源化利用废弃烟梗,从甘薯淀粉加工废水中分离到1株L-乳酸产生菌XJL,并对XJL培养特性、形态特征及生理生化特性进行了检测,同时结合其16S rDNA基因序列同源性分析,初步鉴定为干酪乳杆菌(Lactobacillus casei,L.casei)。XJL能够发酵废弃烟梗提取液(Tobacco stem extraction,TSE)产生L-乳酸。在装载量100 mL/150 mL、接种量7.5%、35℃条件下静置培养48 h,废弃烟梗发酵产L-乳酸的最高值为143 g/kg。

Abstract: To make use of waste tobacco stems, a strain XJL, which could produce L-latic acid via fermentation of tobacco stem extract, was isolated from sweet potato starch waste water. By testing the cultural, morphological, physiological and biochemical characteristics combining with 16S rDNA gene sequence analysis, XJL was preliminarily identified as a strain of Lactobacillus casei. When stem was fermented at 35℃ for 48 hours with a load capacity of 100/150 mL and inoculant rate of 7.5%, the maximum yield of L-lactic acid reached 143 g/kg.