在线凝胶色谱-气质联用测定卷烟主流烟气中的苯并a芘

Determination of Benzoapyrene in Mainstream Cigarette Smoke by Online Gel Permeation Chromatography-Gas Chromatography/Mass Spectrometry

摘要: 建立了准确测定卷烟主流烟气中苯并a芘(BaP)的在线凝胶色谱-气质联用(在线GPC-GC/MS)分析方法。卷烟主流烟气依次经剑桥滤片捕集、环己烷萃取后进行在线GPC-GC/MS测定,以苯并a芘-d₁₂(BaP-d₁₂)为内标进行定量。结果表明:BaP在0.4~50 ng/mL浓度范围内线性关系良好(R²=0.9995),3个加标水平的回收率在95.6%~97.2%之间,平均相对标准偏差(RSD)2.18%,检出限0.28 ng/支。该方法灵敏度高、定性定量结果可靠,适用于卷烟主流烟气中BaP的测定。

Abstract: In order to accurately determine the benzoapyrene (BaP) in mainstream cigarette smoke, an online gel permeation chromatography-gas chromatography/mass spectrometry (online GPC-GC/MS) method was developed. The BaP in mainstream cigarette smoke was collected by a Cambridge filter pad, then the pad was extracted with cyclohexane, the extract was quantitatively analyzed by online GPC-GC/MS with BaP-d₁₂ as an internal standard. The results showed that: BaP displayed good linearity (R²=0.9995) in the range of 0.4 to 50 ng/mL. The recoveries at three spiked levels ranged from 95.6% to 97.2% with the mean relative standard deviation of 2.18% and the limit of detection of 0.28 ng/cig. This method is reliable, sensitive and accurate in qualitative and quantitative analyses, and suitable for the determination of BaP in mainstream cigarette smoke.