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褐变抑制剂对烟叶烘烤过程中酶促棕色化反应的影响

Effects of Browning Inhibitors on Enzymatic Browning Reaction in Tobacco During Flue Curing

  • 摘要: 为研究不同褐变抑制剂对烟叶烘烤过程中酶促棕色化反应的影响,分别采用0.1%柠檬酸+0.05%VC(处理1,T1),0.3%NaHSO3(处理2,T2)和0.2%NaHSO3+0.1%柠檬酸+0.05%VC(处理3,T3)处理采收后的烟叶,清水浸泡作为对照(CK),之后进行烘烤;根据色泽参数(L*,a*b*)、酚类物质含量的变化,评价各处理对酶促棕色化反应的影响,并对实验结果进行相关性分析。结果表明:①在烘烤过程中,烤烟的色泽参数和酚类含量除T1降低外,其他处理都有提高,其中T2提高效果最明显。②L*a*与3个酚类物质参数(总酚、芸香苷和绿原酸含量)极显著正相关。③3种褐变抑制剂都可抑制烟叶烘烤过程中的酶促棕色化反应,其中T2效果最好,T1的效果最差。

     

    Abstract: In order to investigate the effects of different browning inhibitors on enzymatic browning reaction during flue curing,the harvested tobacco leaves were treated with 0.1% citric acid + 0.05% VC(T1),0.3% sodium bisulfite (T2),and 0.2% sodium bisulfite+ 0.1% citric acid+0.05% VC(T3)separately,and the control group(CK)was treated by water immersing,then all of the samples were cured in a curing barn. Color parameters(L*,a*,b*)and phenolic compound contents were used to evaluate the influence of each treatment on enzymatic browning reaction. Meantime the experimental data were correlatively analyzed. The results showed that: 1) The values of color parameters and the contents of phenolic compounds decreased in T1,while increased to a certain extent in T2 and T3, especially in T2. 2)L* and a* extremely significantly positively correlated to total phenolic compound content,rutin content and chlorogenic acid content. 3)All of the three treatments inhibited the enzymatic browning reaction to a certain degree during flue curing,T2 inhibited that the most,T1 the least.

     

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