Abstract: In order to control the growth of tobacco seedlings and provide seedlings suitable for well-cellar transplanting, a contrast experiment of repeating clipping was carried out. The results showed that tobacco seedlings were more suitable for well-cellar transplanting on the third day after the first clipping (only 1/3 to 1/2 of the area of larger leaves was removed) or the second clipping; the restoring period of the transplanted tobacco plants in field was shorter, the amount of dry matter accumulation in tobacco shoot was larger, the agronomic characters, such as plant height, stalk circumference, length and width of the largest leaf and tip, were better, and single leaf weight, yield, output value, average price and percentage of high and middle grade leaf increased. Furthermore, transplanting on the third day after the first clipping could reduce the cost of seedling culturing obviously. Therefore, it is recommended to practice well-cellar transplanting on the third day after the first clipping in flue-cured tobacco production.