Abstract: The contents of cell wall substance, total cellulose, pectin and lignin in 88 samples of flue-cured tobacco from six provinces in China were measured.The findings were as follows:1)the contents of cell wall substance, total cellulose, pectin and lignin were in the ranges of 26%-35%, 13%-18%, 5%-9% and 3.5%-7.5%, respectively;2)there were little differences of cell wall substance, total cellulose, pectin and lignin levels between the same grade tobacco leaves from different areas, except that the tobacco leaf from Bijie City in Guizhou Province showed slightly higher levels and the tobacco leaf from Mile County in Yunnan Province showed slightly low er levels;3)the contents of cell wall substance, total cellulose and pectin were lower in higher grade tobacco of the same cultivar and growing area, while the lignin content was contrary;4)the stalk positions in the order of the content of cell wall substance were lower leaves > upper leaves > cutters, the content of pectin increased and the contents of total cellulose and lignin decreased with the rise of stalk position;5)the color of leaves of the same stalk position in the order of contents of cell wall substances, total cellulose and lignin was lemon > orange, while for pectin, it was contrary;6)the content of cell wall substance in moderate body leaf and that of total cellulose in the le af with thicker body were the lowest, the pectin content increased and lignin content decreased with the increase of tobacco thickness;7)the contents of cell wall substance and total cellulose in the leaves with rich oil were the least and those with poor oil were the highest, the lignin content was slightly increased with the decrease of leaf oil, there was no significant relationship between the pectin content and leaf oil.