烟草SSR反应体系的优化

Optimization of SSR Reaction System in Tobacco

摘要: 以烤烟品种NC2326、G28及其F₁为材料,研究了烟草SSR分析中PCR反应体系的主要成分对SSR扩增结果的影响。结果表明:在总体积为20μL的PCR反应中,含20ng模板DNA,1.5U TaqDNA聚合酶,MgCl₂终浓度为1.5mmol·L^-1,dNTPs浓度为200μmol·L^-1,引物浓度为0.25μmol·L^-1时效果较好。

Abstract: Using flue-cured tobacco cv.NC2326, G28 and their hybrid F₁ as materials, the effects of major elements in PCR reaction system on SSR analysis were studied.The results showed that under the conditions of 1.5U TaqDNA polymerase, terminal MgCl₂ concentration of 1.5mmol·L^-1, 200μmol·L^-1 dNTPs, 0.25μmol·L^-1 primers and 20ng templates of DNA in total volume of 20μL, the result was better in PCR reaction.