陕西烟草病毒病毒原鉴定及种群区系分布

Identification of Tobacco Virus Diseases Pathogen and Distribution of Virus Community in Shaanxi Province

摘要: 2001年在陕西省烟区采集了165个样品,经过生物学测定、血清学反应(琼脂双扩散法和酶联免疫吸附法)和电镜观察,鉴定出5种为害烟草的病毒。其中烟草普通花叶病毒(TMV)34份、烟草蚀纹病毒(TEV)32份、黄瓜花叶病毒(CMV)31份、马铃薯Y病毒(PVY)16份、烟草环斑病毒(TRSV)4份,分别占总标样的20.61%、19.39%、18.79%、9.7%和2.4%。TMV和CMV、TMV和TEV、TMV和PVY复合侵染各为35、2和11份,分别占总标样的21.21%、1.2%和6.67%。TEV和PVY在某些烟区有明显上升的趋势。

Abstract: From 165 tobacco leaf samples collected in the tobacco growing areas of Shaanxi Province in 2001, five viruses, tobacco mosaic virus (TMV), tobacco etch virus (TEV), cucumber mosaic virus (CMV), potato virus Y (PVY)and tobacco ringspot virus (TRSV), were identified through biological determination, serological reaction (agar double diffusion and enzyme-linked immuno-sorbent methods)and electron microscopy observation, and their rates of occurrence were 20.61%, 19.39%, 18.79%, 9.7% and 2.4%, respectively.The complex infection rates of TMV and CMV, TMV and TEV, TMV and PVY were 21.21%, 1.2% and 6.67%, respectively.The infection of TEV and PVY increased obviously in some regions.