烟草粉螟高毒力<i>Bt</i>菌株的发酵条件

烟草粉螟高毒力Bt菌株的发酵条件

摘要: 对烟草粉螟有较高毒力的苏云金杆菌Bt33、Bt53、BtHB 3株菌株的发酵培养条件进行了试验。结果表明:3株菌株的最佳培养温度为30℃、pH值为7.5、转速为180rpm,Bt33、Bt53的最佳培养时间为40h,BtHB为36h,BtHB培养的最佳空气量为19/20(培养容器容积-培养基体积)/培养容器容积,Bt33、Bt53均为9/10。

Abstract: The fermentation conditions of Bt33、Bt53、BtHB isolates of Bacillus thuringiensis, which were high toxicity to Lepidoptera insect, were studied.The results indicated that the optimum culture conditions for all tested isolates were:30℃, pH 7.5, rotating speed 180 rpm.The optimum culture time for Bt33 and Bt53 isolates was 40 hours, while that for BtHB was 36 hours.And the optimum air volume for Bt33 and Bt53 isolates was 9/10 (container volume-medium volume/container volume), while that for BtHB was 19/20.