烟草中乳酸含量的气相色谱/质谱分析

Determination of Lactic Acid in Tobacco by GC/MS

摘要: 以三甲基氢氧化硫为甲酯化试剂,正戊醇为内标,采用INNOWAX毛细管柱(30m×0.25mmi.d.×0.25μm d.f.)和选择离子模式,建立了烟草及烟草制品中乳酸的气相色谱/质谱分析方法。该法的线性范围为9.144×10^-3~121.92×10^-3 mg/mL乳酸(r=0.9986),平均回收率为98.09%,RSD为1.83%。并应用该法测定了部分烟草及烟草制品中乳酸的含量。

Abstract: A method for determining lactic acid in tobacco by GC/MS was established with an Innowax capillary column (30m×0.25mm i.d.×0.25μm d.f.) and selective ion mode, using TMHS as a methyl esterification reagent and n-pentanol as an internal standard.The linear range of this method was from 9.144×10^-3mg/mL to 121.92×10^-3mg/mL lactic acid (r=0.9986).The average recovery of lactic acid was 98.09% and the RSD of this method was 1.83%.The contents of lactic acid in some tobacco leaves and tobacco products were determined with this method.