氨基酸对烟草赤星病菌毒素毒性的影响

Effect of Amino Acids on Tobacco Alternaria alternata Phytotoxin Virulence

摘要: 采用摩擦接种处理法、浇根处理法和发芽种子根处理法测定了烟草赤星病菌毒素的毒性,确定了烟草发芽种子根处理法作为烟草赤星病菌毒素毒性的测定方法。用8种氨基酸分别以5种不同浓度与烟草赤星病菌GL-6毒素混合接种,测定结果表明:L-精氨酸、L-胱氨酸、L-谷氨酸、L-丙氨酸和L-天门冬氨酸所有供试浓度均增强病菌毒素毒性;而L-脯氨酸、L-色氨酸和L-缬氨酸对病菌毒素毒性有抑制作用,其有效浓度分别为0.02~0.2、0.02~0.2和0.02~2mg/mL。

Abstract: The phytotoxin virulence of tobacco Alternaria alternata was tested with three methods, rubbing inoculating, root watering and seed-bud root treating.The seed-bud root treating was chosen as the testing method. Alternaria alternata (GL-6) mixed separately with 8 kinds of amino acid at 5 different concentrations were inoculated.The results indicated that phytotoxin virulence was heightened by l-arginine, l-cystine, l-glutamic acid, l-alanine, and l-aspartic acid at all tested concentrations.However, the phytotoxin virulence could be depressed by lproline, l-tryptophane and l-valine, the effective concentrations were 0.02-0.2mg/mL, 0.02-0.2mg/mL and 0.02-2mg/mL, respectively.