Abstract: The traditional means, such as relative density, refractive index, odor, color, clarity, miscibility, acid value test, and etc., failed to control the quality of tobacco flavor in terms of its constituents.Ten representative constituents in a standard sample and a sample from the same brand flavor purchased at a different time were analy zed and compared by simultaneous distillation and extraction as well as GC/MS.The results showed that the chemistry of tobacco flavor could be effectively monitored by this method and it was also a good reference for cigarette flavoring.